Strategic Transit Automation Research (STAR) Plan 2.0 is a successor to the original 2018 STAR Plan published by FTA. It builds upon the work accomplished from the 2018 STAR Plan (referred to now as STAR Plan 1.0) and provides strategic research and demonstration framework for the next five years to advance driving automation systems that meet public transportation needs. Key components of the plan include conducting enabling research on safe, equitable, and effective transit automation deployments, demonstrating nearly-market-ready prototype technologies in real-world settings, and learning from and sharing knowledge with the transit stakeholder community. Similar to STAR Plan 1.0, STAR Plan 2.0 leverages the core strengths of academia and the public and private sectors and is organized around three complementary work areas: enabling research, integrated demonstrations, and strategic engagement. Efforts under these three areas are designed to complement one another and collectively advance FTA and U.S. Department of Transportation (USDOT) goals in automation.